Coding

Part:BBa_K4632005

Designed by: Haolong Lai   Group: iGEM23_SCAU-China   (2023-10-09)
Revision as of 13:26, 9 October 2023 by LHL scau (Talk | contribs) (→‎Description)


dapA

Description

Diaminopimelic acid is a component of cell walls in certain prokaryotes, especially in the peptidoglycan chains of Gram-negative bacteria. These bacteria require DAP for normal growth. Without DAP, they can still grow but are unable to synthesize the peptidoglycan essential for their cell walls.

Growth of the dapA mutant wm3064 is strictly dependent on exogenously supplied DAP; hence, the removal of DAP provides an efficient counterselection against this donor.(Dehioet al.,1997)

Diaminoheptanedioic acid (DAP) is an α-amino acid. It is an ε-carboxy derivative of lysine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Dehio C, Meyer M. Maintenance of broad-host-range incompatibility group P and group Q plasmids and transposition of Tn5 in Bartonella henselae following conjugal plasmid transfer from Escherichia coli[J]. J BACTERIOL, 1997,179(2):538-540.


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