Part:BBa_K4863002

S-layer protein (Slp) S-layer protein (Slp) is a native protein on the cell surface of cyanobacteria. It is encoded by the gene sll1951 in Synechocystis PCC6803. This protein forms a lattice that is about 30nm wide that covers the cell surface, forming the paracrystalline S-layer. It has an undefined signal peptide at C-terminus.

Fusion of a protein to the N-terminus of Slp is shown to be an effective system for functional surface display since it would yield a cell entirely covered with the fusion protein on the outermost layer.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3112
  Illegal EcoRI site found at 5092
  Illegal XbaI site found at 318
  Illegal PstI site found at 434
  Illegal PstI site found at 4034
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3112
  Illegal EcoRI site found at 5092
  Illegal NheI site found at 741
  Illegal NheI site found at 838
  Illegal PstI site found at 434
  Illegal PstI site found at 4034
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3112
  Illegal EcoRI site found at 5092
  Illegal BglII site found at 2253
  Illegal BamHI site found at 3159
  Illegal BamHI site found at 4473
  Illegal XhoI site found at 376
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3112
  Illegal EcoRI site found at 5092
  Illegal XbaI site found at 318
  Illegal PstI site found at 434
  Illegal PstI site found at 4034
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3112
  Illegal EcoRI site found at 5092
  Illegal XbaI site found at 318
  Illegal PstI site found at 434
  Illegal PstI site found at 4034
  Illegal AgeI site found at 2140
  Illegal AgeI site found at 2323
  Illegal AgeI site found at 2671
  Illegal AgeI site found at 2731
  Illegal AgeI site found at 4252
  
• 1000
  COMPATIBLE WITH RFC[1000]