Coding

Part:BBa_K4632005

Designed by: Haolong Lai   Group: iGEM23_SCAU-China   (2023-10-09)
Revision as of 12:28, 9 October 2023 by LHL scau (Talk | contribs) (→‎Sequence and Features)


dapA

Description

Growth of the dapA mutant wm3064 is strictly dependent on exogenously supplied DAP; hence, the removal of DAP provides an efficient counterselection against this donor.(Dehioet al.,1997)

Diaminoheptanedioic acid (DAP) is an α-amino acid. It is an ε-carboxy derivative of lysine.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 532
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 532
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 532
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 532
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 416
    Illegal EcoRI site found at 532
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Dehio C, Meyer M. Maintenance of broad-host-range incompatibility group P and group Q plasmids and transposition of Tn5 in Bartonella henselae following conjugal plasmid transfer from Escherichia coli[J]. J BACTERIOL, 1997,179(2):538-540.


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