RBS

Part:BBa_K4986001

Designed by: Chan Hoi Huen Heidi   Group: iGEM23_THINKER-CHINA   (2023-10-09)
Revision as of 10:55, 9 October 2023 by Registry (Talk | contribs)

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Suicide system: temperature-controlled promoter +SRRZ cutting gene

Considering the need to achieve sufficient efficacy without causing harm to Escherichia coli (E. coli), we have decided to design a targeted suicide gene strategy focused on the bacterial cell wall. The suicide gene system we are utilizing comprises a series of linked genes known as SRRz. The products of the R and RZ genes within this system are primarily responsible for degrading the bacterial cell wall. Furthermore, since this gene chain exclusively targets cell wall degradation, it poses no harm to human cells, thereby aligning with biological safety requirements.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 388
    Illegal BsaI site found at 799


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Parameters
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