DNA

Part:BBa_K4621029

Designed by: Ruikang Chen   Group: iGEM23_SCUT-China   (2023-10-01)
Revision as of 09:08, 1 October 2023 by Registry (Talk | contribs)

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Spacer of 62-sgRNA to target GQS52_08040

This part contains the spacer sequence used to target a specific DNA fragment of a homoserine dehydrogenase gene, GQS52_08040 in SCUT-3. It must be co-expressed with a sgRNA that has a 20-base pair complementary sequence to the target gene that dCas9 can effectively silence the gene of interest [1]. In our case, we used it to target the binding site of RNP to effectively repress the transcription of the target gene. As a result, the production of the target product is intricately regulated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None