Regulatory

Part:BBa_K203106

Designed by: Corinna Hiller and Hannah Uckelmann   Group: iGEM09_Heidelberg   (2009-10-15)
Revision as of 08:47, 19 October 2009 by Chiller (Talk | contribs)

HMG-CoA synthase promoter (sterol regulated)

This part is a natural promoter responsive to...... and when placed upstream of a gene makes it regulatable by the elements to which this promoter is sensitive.


Usage and Biology

3-hydroxy-3-methylglutaryl-CoA synthase (HMG CoA synthase) is an enzyme catalyzing the condensation of Acetyl-CoA and acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). There are two distinct HMG CoA synthases, the cytosolic and the mitochondrial form, encoded by two different genes. The reaction catalyzed by the cytosolic enzyme is a part of the biosynthesis of cholesterol. In mitochondria the same reaction is responsible for keton body formation. Sterol regulatory elements in the promoter region of the gene are responsible for transcriptional regulation [40].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

n/aHMG-CoA synthase promoter (sterol regulated)
[[Image:|thumb|none|350px|BBa_K203106 is a natural HMG CoA synthase promoter induced approximately by ?% by Lipoprotein Deficient Serum;. [http://2009.igem.org/Team:Heidelberg/Measurement REU] was determined by TECAN, 1 experiment à three replicates]]


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Categories
Parameters
n/aHMG-CoA synthase promoter (sterol regulated)