Composite

Part:BBa_K4814013

Designed by: CHIEN-YUEH LIU   Group: iGEM23_PuiChing-Macau   (2023-09-28)
Revision as of 01:36, 29 September 2023 by Registry (Talk | contribs)

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RecA(K3)-Strong-eGFP

To optimize the design of the RecA-eGFP reporter (BBa_K3020000), we replaced the original B0034 RBS with a new RBS designed by Zhang, M. et al (2022), and see if the effectiveness will increase. However, from our experimental result, using RBS BBa_K4814012 does not enhance the level of fluorescence of the reporter.

Zhang, M., Holowko, M. B., Zumpe, H. H., & Ong, C. S. (2022). Machine Learning Guided Batched Design of a Bacterial Ribosome Binding Site. ACS Synthetic Biology, 11(7), 2314-2326. https://doi.org/10.1021/acssynbio.2c00015

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 847
  • 1000
    COMPATIBLE WITH RFC[1000]


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