Coding
Ble

Part:BBa_K5023000

Designed by: Lucas Daniel Ovelar Vargas   Group: iGEM23_UNILA-LatAm   (2023-10-08)
Revision as of 18:47, 8 October 2023 by Registry (Talk | contribs)

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Bleomycin Resistance Gene

Resistance to bleomycin is conferred by the product of the Sh ble gene, which was first isolated from Streptoalloteichus hindustanus. The Sh ble gene product binds to the antibiotic in a one-to-one ratio, preventing it from causing DNA cleavage. This resistance gene is utilized as a selectable marker in certain cloning and expression vectors where bleomycin or its analogs, like Zeocin, are used as the antibiotic for selection.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 227
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 227
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 227
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 227
    Illegal NgoMIV site found at 608
    Illegal NgoMIV site found at 669
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None