Coding

Part:BBa_K4613002

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-08)
Revision as of 17:52, 8 October 2023 by Registry (Talk | contribs)

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ADH3

ADH3 is an amidohydrolase derived from Stenotrophomonas acidaminiphila and forms an octamer in solution. ADH3 was reported to exhibit 57- to 35,000-fold higher activity than other enzymes and is the most efficient OTA-detoxifying enzyme reported thus far and can hydrolyze OTA to nontoxic ochratoxin α (OTα) and L-β-phenylalanine (Phe). Moreover, soluble protein expression of ADH3 in Escherichia coli has been realized.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 733
    Illegal AgeI site found at 421
    Illegal AgeI site found at 583
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None