Coding

Part:BBa_K4613001

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-09-29)
Revision as of 06:46, 29 September 2023 by Registry (Talk | contribs)

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Mature Carboxypeptidase A（M-CPA）

Carboxypeptidase A（CPA), derived from bovine pancreas, is a 47 kDa zinc-dependent metal carboxypeptidase. Mature Carboxypeptidase A(M-CPA) removed the signal peptide composed of 16 amino acid residues and the leading peptide composed of 94 amino acid residues and contains a mature peptide of composed of 309 amino acid residues(35kDa). Mature Carboxypeptidase A has an efficient capacity to hydrolyze OTA into the non-toxic product ochratoxin α and L-α-phenylalanine (Phe). The degradation rate was up to 93.36%. Compared with commercial CPA(S-CPA), M-CPA obtained better thermal tolerance and stability at a wider range of pH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 88
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 198
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None