Part:BBa_K4585007

pcDNA3.1(+)-3xHA-Gal4-KRAB-NLS linearized vector

The purpose of the linear vector is to perform homologous recombination with the KRAB homologous recombination fragment (BBa _ K4585003) to obtain the target product pcDNA3.1 (+) -3 HA-Gal 4-KRAB-NLS plasmid.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 46
  Illegal XbaI site found at 85
  Illegal SpeI site found at 4849
  Illegal PstI site found at 51
  Illegal PstI site found at 1480
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 46
  Illegal SpeI site found at 4849
  Illegal PstI site found at 51
  Illegal PstI site found at 1480
  Illegal NotI site found at 72
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 46
  Illegal BglII site found at 4612
  Illegal BamHI site found at 6048
  Illegal XhoI site found at 79
  Illegal XhoI site found at 5818
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 46
  Illegal XbaI site found at 85
  Illegal SpeI site found at 4849
  Illegal PstI site found at 51
  Illegal PstI site found at 1480
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 46
  Illegal XbaI site found at 85
  Illegal SpeI site found at 4849
  Illegal PstI site found at 51
  Illegal PstI site found at 1480
  Illegal NgoMIV site found at 590
  Illegal NgoMIV site found at 1931
  Illegal NgoMIV site found at 2216
  Illegal AgeI site found at 143
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 5737
  Illegal BsaI.rc site found at 3744
  Illegal BsaI.rc site found at 5482
  Illegal SapI site found at 2661
  Illegal SapI.rc site found at 7
  Illegal SapI.rc site found at 1780
  Illegal SapI.rc site found at 1990