Composite

Part:BBa_K4814014

Designed by: CHIEN-YUEH LIU   Group: iGEM23_PuiChing-Macau   (2023-09-28)
Revision as of 12:19, 3 October 2023 by Ginaliu (Talk | contribs)

RecA(K6)-B0034-eGFP

Hoping to validate previous iGEM team’s result and investigate the impact of the genotoxicity of carcinogens, we conducted dosage dependent tests on the top10 E.coli bacteria. RecA(K3), used with eGFP (BBa_K302002) as bioreporter to detect DNA damaging agents such as UV, H2O2 and nalidixic acid, is the promoter derived from BBa_K3020001 by team BIT 2019. This promoter is an optimized version of BBa_K629001 (K6), which is a design of team SYSU 2011 aimed to drive the motor system under a radioactive environment.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 881
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 839
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None