Coding

Part:BBa_K4632002

Designed by: Haolong Lai   Group: iGEM23_SCAU-China   (2023-10-08)
Revision as of 14:46, 8 October 2023 by LHL scau (Talk | contribs)


Cry3A-like toxin


Bacillus thuringiensis UTD-001, as well as the protoxin and toxin of this isolate, could be used to control pests such as fire ants, carpenter ants, Argentine ants, and Pharaoh's ants, including Solenopsis invicta(S. Invicta). Cry3A-like toxin isolated fromUTD-001 have been shown to be toxic to S.Invicta.(Bulla and Candas, 2003)


How does it work?

It has been shown that after treatment with papain in vitro, the Cry3A-like toxin prototoxin (72.9 KD) forms an active toxin (66.6 KD) that is toxic to S. Invicta.(Bulla and Canda, 2003)


B. '''Eco-friendly and Safe''' ---- Bt (Bacillus thuringiensis) is widely recognized as a safe and environmentally benign insecticide. And the Bt toxin Cry3A-like protein we used is Eco-friendly and Safe.(see more detail on [https://2023.igem.wiki/scau-china/safety]; in a pSB1C3 (already in iGEM biobrick format), without promotor

  • : in a pBAD18, under the control of the arabinose inductible promotor pBAD.
    • 666 Sequence and Features BBa_K4632002 SequenceAndFeatures

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    Parameters
    strainE.coli BL21(DE3)