Part:BBa_K4719028

phaC-phaA-phaB-pKARA_RT3 operon for in situ dyed bacterial cellulose - polyhydroxybutyrate composite
Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 4992
Illegal SpeI site found at 37
Illegal PstI site found at 824
Illegal PstI site found at 1397
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 4992
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37
Illegal PstI site found at 824
Illegal PstI site found at 1397
Illegal NotI site found at 200
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 4992
Illegal BglII site found at 642
Illegal BamHI site found at 3039
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 4992
Illegal SpeI site found at 37
Illegal PstI site found at 824
Illegal PstI site found at 1397
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 4992
Illegal SpeI site found at 37
Illegal PstI site found at 824
Illegal PstI site found at 1397
Illegal NgoMIV site found at 253
Illegal NgoMIV site found at 368
Illegal NgoMIV site found at 602
Illegal NgoMIV site found at 914
Illegal NgoMIV site found at 1193
Illegal NgoMIV site found at 1606
Illegal NgoMIV site found at 1673
Illegal AgeI site found at 341
Illegal AgeI site found at 4455
1000
COMPATIBLE WITH RFC[1000]

Possible application as colorful biodegradable plastic.

Growth burden

In order to work with E. coli for designing constructs and testing synthetic biology systems, the growth burden of said synthetic biology tools has to be measured. We performed growth burden evaluation by measuring OD600 for five hours of modified and unmodified E. coli DH5α. The composite of in situ dyed PHB did not inhibit the growth of E. coli as seen in Figure 1.