Composite

Part:BBa_K4604023

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-07)
Revision as of 21:12, 7 October 2023 by Registry (Talk | contribs)

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piG_21 (tetR_bluB_riboK12_mazF_AmpProm_mazE)

BioBrick piG_20 is a plasmid consisting of the tet promoter/repressor, bluB, an AdoCbl riboswitch, MazF, the rrnB terminator, the Amp promoter, MazF and the rrnB terminator. The backbone we used in the experiments is pGGAselect.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
    Illegal BamHI site found at 2756
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1603
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2238
    Illegal BsaI site found at 2484
    Illegal BsaI site found at 3045


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Categories
Parameters
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