Coding
Part:BBa_K4645003
Designed by: Zekun Luo Group: iGEM23_HZAU-China (2023-10-07)
Fusion protein formed by CsgA and Hsa with (GGGGS)4 as linker.
The NR2 domain of Hsa is the sialic-binding core of Hsa protein, and CsgA is the extracellular fiber component of Escherichia coli biofilm formation. The two proteins were connected with a flexible linker to enable them to colonize on cat teeth and form a stable biofilm, creating conditions for the realization of subsequent neutralization.A 6x His tag was added to the C-terminus of the protein for purification or specificity testing in experiments.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 37
Illegal PstI site found at 861 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 37
Illegal PstI site found at 861 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 37
Illegal PstI site found at 861 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 37
Illegal PstI site found at 861 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |