Part:BBa_K4767009

PluxⅠ-RBS-luxR(△2-162)-RBS-cymA-TT

Uses a factor P_luxⅠ(BBa_R0062) ,strong RBS(BBa_J34801), luxR(△2-162)(BBa_K4767000), cymA(BBa_K2706008)and TT(BBa_B015). This composite part is a BioBrick.

Usage and Biology

This is the response part of the luxR amplifier with the cymA gene as the reporter. In our design, if luxR(△2-162) was expressed as a result of responding, this part can be activated to express cymA and luxR(△2-162). CymA will increase the ability for the Shewanella oneidensis to transfer the electrons, and luxR(△2-162) will functions in a positive feedback loop as it can bind to the P_luxⅠ promoter and activate its own transcription.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 685
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.