Part:BBa_K4727003
M13 standard phagemid
This part, is a new backbone intended to be useful in the production of M13 bacteriophages carrying a genome of desire. It has been developed starting from a commercially available phagemide (pTZ19R) that has been deleted of all the prohibited restriction sites. Following this, BioBrick RFC[10] standard prefix and suffix were added. Any part of interest can now be easily cloned between the prefix and suffx in order to trasduce the cell if interest. It has been developed with the aim of being used together with part BBa_K4727002 or any other M13 helper phage. The phagemid and the helper plasmid must be co-transformed in E. coli, thus allowing the production of phage particles. Its inability to propagate within host cells due to the absence of phage protein encoding genes, ensures the prevention of unintended dissemination of engineered constructs. Whereas, its adaptability is a remarkable feature - it can be easily tailored to the specifics of individual cases, thus presenting a versatile and readily adjustable platform tailored to different purposes.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 623
Illegal suffix found at 645 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 623
Illegal SpeI site found at 646
Illegal PstI site found at 660
Illegal NotI site found at 629
Illegal NotI site found at 653 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 623 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 623
Illegal suffix found at 646 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 623
Plasmid lacks a suffix.
Illegal XbaI site found at 638
Illegal SpeI site found at 646
Illegal PstI site found at 660
Illegal NgoMIV site found at 127 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2026
Illegal SapI site found at 943
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