Composite

Part:BBa_K4767005

Designed by: Fangjun Liu   Group: iGEM23_CUG-China   (2023-10-04)
Revision as of 06:01, 7 October 2023 by Xiang li8 (Talk | contribs) (→‎Usage and Biology)


PluxⅠ-RBS-luxR(△2-162)-RBS-gfp-TT

Uses a factor PluxⅠ(BBa_R0062) ,strong RBS(BBa_J34801), luxR(△2-162)(BBa_K4767000) and gfp coding region(BBa_E0040)and TT(BBa_B015). This part is an easy BioBrick.


Usage and Biology

This is the response part of the luxR amplifier with the gfp gene as the reporter. In our design, if luxR(△2-162) was expressed as a result of responding, this part can be activated to express gfp and luxR(△2-162), and luxR(△2-162) will functions in a positive feedback loop as it can bind to the PluxI promoter and activate its own transcription.

File:BBa K4767005.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1021

References

Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.


[edit]
Categories
//classic/reporter
Parameters
biologyVibrio fischeri