Coding

Part:BBa_K4591002

Designed by: Pengyu Rao   Group: iGEM23_ZJUT-China   (2023-05-10)
Revision as of 05:11, 8 October 2023 by Kaga (Talk | contribs)


XylSmut

ZJUT-China 2023's Contribution

Usage and Biology

XylS is an archetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-terminal domain (CTD) involved in DNA binding, and an N-terminal domain required for effector binding and protein dimerization. XylS detect benzoate and its derivatives, mainly methyl and chlorine substitution at 2-, 3- carbon[1].

In this way, the Xyl could active the Pm promotor by recognizes two 15-bp repeats (TGCA-N6-GGNTA) in Pm promoter when the benzoate and its derivatives was detected.

The xylsmut is upgraded form an existing part Xyls(BBa_K108029)from the Team iGEM_Tsinghua.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None