Composite

Part:BBa_K4604017:Design

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-07)
Revision as of 15:00, 7 October 2023 by Registry (Talk | contribs)

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piG_02a (leaky_tetR_riboK12_mazF_mTurq)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal AgeI site found at 1391
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2265


Design Notes

A GS-linker had to be cloned between MazF and mTurquoise to ensure correct folding and functionality of both proteins. A FLAG-tag was fused to MazF to make it detectable in western blot.


Source

Assembled with Golden Gate.

References