Coding
OleP

Part:BBa_K4759001:Design

Designed by: Jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-09-02)
Revision as of 14:24, 7 October 2023 by JianghuaChen (Talk | contribs) (→‎References)


OleP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 16
    Illegal AgeI site found at 114
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Co-expressed with other components，and Olep itself has not changed


Source

a gene coding for multifunctional bacterial P450 monooxygenase CYP107D1(Olep)


References

[1] G P, I F, C E, et al. Dissecting the Cytochrome P450 OleP Substrate Specificity: Evidence for a Preferential Substrate[J]. Biomolecules, 2020, 10(10). [2] B H, H Y, J Z, et al. Whole-Cell P450 Biocatalysis Using Engineered Escherichia coli with Fine-Tuned Heme Biosynthesis[J]. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2023, 10(6): e2205580