Part:BBa_K4897001
BS DNA-162 (BS DNA 2.0) using in L-RCA for detecting P. acne
BS DNA-162 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. The binding region is the key element in reacting with the P. acne 16s rRNA gene. The DNA ligase will perform the ligation of the single-stranded DNA meanwhile the phi29 will generate double-stranded DNA through amplification primers
Usage and Biology
BS DNA-162 would bind the bacterial DNA of P. acne and show great sensitivity and specificity.
Characterization
<img src=" " width="200" height="auto" /> |
| Fig.1 Characterization |
Lane 1 is a 100 bp DNA ladder. Lane 2 is a positive control tested by synthetic and isolated P. acne DNA. Lane 3 is a positive result tested by samples from human faces through washing.
The positive control and the positive result both have amplification results beyond 162, the initial size, suggesting successful DNA ligation and amplification.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 83
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 57
Illegal SpeI site found at 83 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 68
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 83
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 83
- 1000COMPATIBLE WITH RFC[1000]
| None |