Part:BBa_K4897001

BS DNA-162 (BS DNA 2.0) using in L-RCA for detecting P. acne

BS DNA-162 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. The binding region is the key element in reacting with the P. acne 16s rRNA gene. The DNA ligase will perform the ligation of the single-stranded DNA meanwhile the phi29 will generate double-stranded DNA through amplification primers

Usage and Biology

BS DNA-162 would bind the bacterial DNA of P. acne and show great sensitivity and specificity.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 83
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 57
  Illegal SpeI site found at 83
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 68
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 83
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 83
  
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization