Intermediate
Part:BBa_K260015:Design
Designed by: Kaj Bernhardt Group: iGEM09_BIOTEC_Dresden (2009-10-18)
P_FRT_dhfr. (strong promoter, translated FRT site, trimethoprim resistance)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 79
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 79
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 79
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A strong promoter was necessary and we use [[Part:BBa_J23100|]]. An appropriate reading frame for the FRT site had to be found to prevent premature Stop-codons and hydrophobic amino acid residues. Further, the gene encoding TmpR was codon-optimised.
Source
This part was synthesised by "Mr Gene" (Geneart) and is available in the following plasmid backbones:
[[Part:BBa_K260004|]]: pMA-@CC2FosMB-00500bp
[[Part:BBa_K260005|]]: pMA-@CC2FosMB-01000bp
[[Part:BBa_K260006|]]: pMA-@CC2FosMB-02000bp
[[Part:BBa_K260007|]]: pMA-@CC2FosMB-05000bp
[[Part:BBa_K260008|]]: pMA-RQ-@CC2FosMB-10000bp