Part:BBa_K200013

Opiorphin + EK cleavage site

Background

Opiorphin is a pentapeptide (five amino acids long) that was isolated from human saliva in 1996. Opiorphin acts as a pain killing agent. The amino acid sequence for opiorphin is Glutamine-Arginine-Phenylalanine-Serine-Arginine.

A combination of opiorphin's potency and natural presence in saliva have sparked a great deal of interest from a number of philosophical movements most notably from [http://en.wikipedia.org/wiki/David_Pearce_(philosopher) David Pearce] who belongs to the [http://en.wikipedia.org/wiki/Utilitarianism#Negative negative utilitarian] school of ethics.

Mechanism of Action

Preliminary rat studies indicate that opiorphin has a pain killing effect six times stronger than that of morphine. With regards to its mechanism of action, opiorphin prevents the breakdown of enkephalins which are natural pain-killing opioids.

EK Cleavage

See 'Design Notes' for our rationale behind incorporating an EK cleavage sequence on the N terminus of the opiorphin pentapeptide.

Side Effects

Priapism:

A recent study involved genetically engineering retired breeder rats to constitutively express opiorphin. Interestingly, this resulted in the development of a priapic-like condition. It is thought that this condition resulted from the upregulation of the ornithine decarboxylase gene (ODC) by opiorphin ¹.

Antidiarrheal:

In an in-vitro bioassay it was shown that opiorphin (in concentarions 10⁻⁶ to 10⁻⁴ M) caused colonic contraction in a concentration-dependent manner ². Based on this evidence, opiorphin could have potential as an antidiarrheal agent.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

<biblio>

1. 1 pmid=19657052

1. 2 pmid=19442408

</biblio>