Coding

Part:BBa_K4989001:Design

Designed by: Athanasia Arampatzi   Group: iGEM23_Thrace   (2023-10-07)
Revision as of 10:59, 7 October 2023 by Registry (Talk | contribs)

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3-hydroxybutyryl-CoA dehydrogenase (bhbd)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence that we designed has a start and ending codon and a single open reading frame (ORF). Also, the sequence is expected to be expressed in our hosts Lactobacillus rhamnosus GG, Lactobacillus plantarum LP-01, Lactobacillus acidophilus DSM 20079 and E.coli K-12 and thus was codon-optimized.


Source

Our part was synthetically produced but the sequence comes from Coprococcus sp. L2-50 DSM.


References