Part:BBa_K4813000
dTomato fluoresence protein Codon Optimized for E coli
The coding sequence mentioned encodes a protein called dTomato, which produces orange fluorescence. It forms a dimer and has a molecular weight of around 27.0 kDa. This protein originates from a species called Discosoma.
To enhance its performance for our project, we used IDT codon optimization tools to optimize the sequence. We incorporated this optimized sequence into two composite parts. One part served as a reporter gene for detecting formaldehyde (pFrmR formaldehyde sensing), while the other part acted as a positive control, where the expression of dTomato was driven by a strong and consistent promoter J23100.
Despite previous literature suggesting that tdTomato exhibits better expression and brighter light emission compared to dTomato, our experimental results demonstrated that dTomato displayed a significantly brighter red color than tdTomato in our positive controls. As a result, we selected dTomato for the final assay to effectively detect the presence of formaldehyde.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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