Composite
Part:BBa_K4767017
Designed by: Fangjun Liu Group: iGEM23_CUG-China (2023-10-04)
Pbad-RBS-luxI-TT-Ptac-luxR-TT-PluxI-gfp-TT
Uses a factor PluxⅠ(BBa_R0062) ,strong RBS(BBa_J34801), luxⅠ(luxI C0061) ,gfP coding region(BBa_E0040), Ptac(M31370), luxR(BBa_C0062)and Pbad(Ara K808000). This part is an easy BioBrick.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1872
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3021
Illegal BsaI.rc site found at 3728
Illegal SapI site found at 961
[edit]
Categories
Parameters
//classic/reporter
biology |