Composite
Part:BBa_K4767012
Designed by: Fangjun Liu Group: iGEM23_CUG-China (2023-10-04)
Pars-RBS-arsR-RBS-luxR(△2-162)-TT-PluxⅠ-RBS-luxR(△2-162)-RBS-mtrC -TT
Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor arsR(BBa_J15101), luxR(△2-162)(BBa_K4767000) TT(BBa_B0015) ,PluxⅠ(BBa_R0062) and mtrC (BBa_K3102020).This composite part is used in the iron reduction experiment and MFC.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 270
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1543
Illegal BsaI.rc site found at 929
[edit]
Categories
Parameters
//classic/reporter
biology | Escherichia coli & Vibrio fischeri & Shewanella oneidensis |