Reporter

Part:BBa_K274002:Design

Designed by: Shuna Gould   Group: iGEM09_Cambridge   (2009-10-08)
Revision as of 13:16, 18 October 2009 by Shuna (Talk | contribs) (→‎Source)

Vio operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1029
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5602
    Illegal BamHI site found at 4289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3814
    Illegal AgeI site found at 4010
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 6552
    Illegal SapI.rc site found at 6627


Design Notes

We added complementary restriction sites on either side of vioC and vioD (BamHI, BglII, HindIII). This allows them to be seperately removed to produce more colours from the operon.


Source

The VioA-E genes were originally from from Chromobacterium voilaceum ATCC 12472 in the pPSX vio+ plasmid. This was kindly provided by John Pemberton; Department of Microbiology and parasitology, University of Queensland, Brisbane, Australia. (Sarovich & Pemberton (2007) Plasmid 57:306-313). The completed gene was designed using GeneDesigner program from DNA2.0 and the codons optimised for E. coli with the help of DNA2.0 (Welch, Villalobos, Gustafsson and Minshull (2009) J. R. Soc. Interface)

References