Part:BBa_K239001

Spy promoter, activated by Phosporylated CpxR, BaeR and Sigma70

Sequence contains 2 phosphorylated BaeR (Bacterial Adaptative rEsponse) binding sites, 1 phosphorylated CpxR (Conjugative Plasmid eXpression) binding site and transcription is initiated by sigma factor 70. Experiments by Bury-Moné et al. (2009) indicates that also RcsB (Regulator of colanic acid Capsule Synthesis) induces expression of the Spy gene.

Function: Can detect various periplasmic stresses such as Copper (4mM CuCl2), Indole (4mM) and Ethanol (5%). We hope that it also can be used to detect presence of relevant unfolded proteins in the periplasmic space (e.g. target pharmaceuticals), to detect shear stress or other so far undefined relevant stress.

Note: DegP is dependent of Sigma E and can not be induced only by over expression of CpxR. In comparison, the Spy promoter, which is dependent on sigma factor 70, can be induced up to 40 fold by over expression of CpxR. (Bury-Moné et al. 2009)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]