Composite

Part:BBa_K4800008

Designed by: Yifei Wu   Group: iGEM23_NJTech-China-B   (2023-10-06)
Revision as of 17:04, 6 October 2023 by Registry (Talk | contribs)

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Ptrc-sfp-MmCAR(Q302E)-SpyTag-EutM-SpyCatcher-YahK

Ptrc is the promoter, YciA-sfp-Q302E is a mutant gene derived from BBa_K1655000, EutM is the protein scaffold, Yahk is the aldo-keto reductase, and 'molecular glue' SpyTag/SpyCatcher can be used to construct multi-enzyme complexes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1313
    Illegal EcoRI site found at 5098
    Illegal XbaI site found at 5016
    Illegal SpeI site found at 527
    Illegal PstI site found at 1748
    Illegal PstI site found at 1775
    Illegal PstI site found at 3044
    Illegal PstI site found at 3071
    Illegal PstI site found at 3464
    Illegal PstI site found at 3960
    Illegal PstI site found at 4604
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1313
    Illegal EcoRI site found at 5098
    Illegal NheI site found at 5786
    Illegal SpeI site found at 527
    Illegal PstI site found at 1748
    Illegal PstI site found at 1775
    Illegal PstI site found at 3044
    Illegal PstI site found at 3071
    Illegal PstI site found at 3464
    Illegal PstI site found at 3960
    Illegal PstI site found at 4604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1313
    Illegal EcoRI site found at 5098
    Illegal BglII site found at 2302
    Illegal BglII site found at 2928
    Illegal BglII site found at 3204
    Illegal BglII site found at 3895
    Illegal BamHI site found at 1285
    Illegal BamHI site found at 4859
    Illegal BamHI site found at 5092
    Illegal XhoI site found at 4844
    Illegal XhoI site found at 4931
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1313
    Illegal EcoRI site found at 5098
    Illegal XbaI site found at 5016
    Illegal SpeI site found at 527
    Illegal PstI site found at 1748
    Illegal PstI site found at 1775
    Illegal PstI site found at 3044
    Illegal PstI site found at 3071
    Illegal PstI site found at 3464
    Illegal PstI site found at 3960
    Illegal PstI site found at 4604
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1313
    Illegal EcoRI site found at 5098
    Illegal XbaI site found at 5016
    Illegal SpeI site found at 527
    Illegal PstI site found at 1748
    Illegal PstI site found at 1775
    Illegal PstI site found at 3044
    Illegal PstI site found at 3071
    Illegal PstI site found at 3464
    Illegal PstI site found at 3960
    Illegal PstI site found at 4604
    Illegal NgoMIV site found at 3572
    Illegal NgoMIV site found at 3634
    Illegal NgoMIV site found at 3851
    Illegal NgoMIV site found at 4298
    Illegal AgeI site found at 1478
    Illegal AgeI site found at 2489
    Illegal AgeI site found at 2786
    Illegal AgeI site found at 3671
    Illegal AgeI site found at 3680
    Illegal AgeI site found at 4355
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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