![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4800008
Designed by: Yifei Wu Group: iGEM23_NJTech-China-B (2023-10-06)
Ptrc-sfp-MmCAR(Q302E)-SpyTag-EutM-SpyCatcher-YahK
Ptrc is the promoter, YciA-sfp-Q302E is a mutant gene derived from BBa_K1655000, EutM is the protein scaffold, Yahk is the aldo-keto reductase, and 'molecular glue' SpyTag/SpyCatcher can be used to construct multi-enzyme complexes.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1313
Illegal EcoRI site found at 5098
Illegal XbaI site found at 5016
Illegal SpeI site found at 527
Illegal PstI site found at 1748
Illegal PstI site found at 1775
Illegal PstI site found at 3044
Illegal PstI site found at 3071
Illegal PstI site found at 3464
Illegal PstI site found at 3960
Illegal PstI site found at 4604 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1313
Illegal EcoRI site found at 5098
Illegal NheI site found at 5786
Illegal SpeI site found at 527
Illegal PstI site found at 1748
Illegal PstI site found at 1775
Illegal PstI site found at 3044
Illegal PstI site found at 3071
Illegal PstI site found at 3464
Illegal PstI site found at 3960
Illegal PstI site found at 4604 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1313
Illegal EcoRI site found at 5098
Illegal BglII site found at 2302
Illegal BglII site found at 2928
Illegal BglII site found at 3204
Illegal BglII site found at 3895
Illegal BamHI site found at 1285
Illegal BamHI site found at 4859
Illegal BamHI site found at 5092
Illegal XhoI site found at 4844
Illegal XhoI site found at 4931 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1313
Illegal EcoRI site found at 5098
Illegal XbaI site found at 5016
Illegal SpeI site found at 527
Illegal PstI site found at 1748
Illegal PstI site found at 1775
Illegal PstI site found at 3044
Illegal PstI site found at 3071
Illegal PstI site found at 3464
Illegal PstI site found at 3960
Illegal PstI site found at 4604 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1313
Illegal EcoRI site found at 5098
Illegal XbaI site found at 5016
Illegal SpeI site found at 527
Illegal PstI site found at 1748
Illegal PstI site found at 1775
Illegal PstI site found at 3044
Illegal PstI site found at 3071
Illegal PstI site found at 3464
Illegal PstI site found at 3960
Illegal PstI site found at 4604
Illegal NgoMIV site found at 3572
Illegal NgoMIV site found at 3634
Illegal NgoMIV site found at 3851
Illegal NgoMIV site found at 4298
Illegal AgeI site found at 1478
Illegal AgeI site found at 2489
Illegal AgeI site found at 2786
Illegal AgeI site found at 3671
Illegal AgeI site found at 3680
Illegal AgeI site found at 4355 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |