Composite

Part:BBa_K4800018

Designed by: Qiming Zou   Group: iGEM23_NJTech-China-B   (2023-10-06)
Revision as of 15:06, 6 October 2023 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Ptrc-sfp-MmCAR(Q302E)-YahK

The promoter was switched to Ptrc, which can be expressed in Escherichia coli KA30, and YciA-sfp-Q302E was derived from BBa_K1655000, an acid-base mutation of carboxylic acid reductase MmCAR at position 302, which replaces glutamine with glutamic acid to improve the efficiency of carboxylic acid reduction, and YahK, an aldehyde reductase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1313
    Illegal SpeI site found at 527
    Illegal PstI site found at 1748
    Illegal PstI site found at 1775
    Illegal PstI site found at 3044
    Illegal PstI site found at 3071
    Illegal PstI site found at 3464
    Illegal PstI site found at 3960
    Illegal PstI site found at 4604
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1313
    Illegal NheI site found at 4872
    Illegal SpeI site found at 527
    Illegal PstI site found at 1748
    Illegal PstI site found at 1775
    Illegal PstI site found at 3044
    Illegal PstI site found at 3071
    Illegal PstI site found at 3464
    Illegal PstI site found at 3960
    Illegal PstI site found at 4604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1313
    Illegal BglII site found at 2302
    Illegal BglII site found at 2928
    Illegal BglII site found at 3204
    Illegal BglII site found at 3895
    Illegal BamHI site found at 1285
    Illegal XhoI site found at 4844
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1313
    Illegal SpeI site found at 527
    Illegal PstI site found at 1748
    Illegal PstI site found at 1775
    Illegal PstI site found at 3044
    Illegal PstI site found at 3071
    Illegal PstI site found at 3464
    Illegal PstI site found at 3960
    Illegal PstI site found at 4604
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1313
    Illegal SpeI site found at 527
    Illegal PstI site found at 1748
    Illegal PstI site found at 1775
    Illegal PstI site found at 3044
    Illegal PstI site found at 3071
    Illegal PstI site found at 3464
    Illegal PstI site found at 3960
    Illegal PstI site found at 4604
    Illegal NgoMIV site found at 3572
    Illegal NgoMIV site found at 3634
    Illegal NgoMIV site found at 3851
    Illegal NgoMIV site found at 4298
    Illegal AgeI site found at 1478
    Illegal AgeI site found at 2489
    Illegal AgeI site found at 2786
    Illegal AgeI site found at 3671
    Illegal AgeI site found at 3680
    Illegal AgeI site found at 4355
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None