Coding

Part:BBa_K4665004

Designed by: Fien Eickmans   Group: iGEM23_MSP-Maastricht   (2023-09-29)
Revision as of 07:09, 29 September 2023 by Registry (Talk | contribs)

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SazCA membrane fusion protein

This sequence codes for a membrane fusion protein that displays SazCA outside of the bacterial cell membrane. The fusion protein is composed of three parts. The first part is the N-terminal of the ice nucleation protein derived from Pseudomonas syringae, which will be embedded in the outer membrane of E. coli (BBa_K4665001). The second part consists of a flexible linker composed of 4 glycine repeats followed by a serine amino acid (G4S linker; BBa_K2549053), which is used to connect the N-terminal of the INP to the SazCA enzyme (BBa_K4665120).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None