Composite

Part:BBa_K4604015:Design

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-01)
Revision as of 10:31, 1 October 2023 by Registry (Talk | contribs)

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piG_01b (tetR_bluB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1618


Design Notes

The start codon of the bluB was changed to ATG to act as a start for translation in E. coli. Correct overhangs had to be designed to ensure high efficiency with Golden Gate.



Source

Assembled with Golden Gate cloning.

References