Composite

Part:BBa_K4800009

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-06)
Revision as of 15:42, 6 October 2023 by Registry (Talk | contribs)

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davB-davA-GabT

The fragment expresses L-lysine monooxygenase, 5-aminopentanamidase, and 4-aminobutyrate aminotransferase GabT, respectively, which catalyze the reactions: L-lysine + dioxygen → 5-aminopentanamide + CO2 + H2O, 5-aminopentanamide + H2O → 5-aminopentanoate + ammonium 5-aminopentanoate + 2-oxoglutarate ↔ L-glutamate + glutarate semialdehyde Constitutes the Lysine to 5-oxopentanoate pathway

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 269
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1358
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1518
    Illegal AgeI site found at 925
    Illegal AgeI site found at 1174
    Illegal AgeI site found at 2896
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None