![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4800009
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-06)
davB-davA-GabT
The fragment expresses L-lysine monooxygenase, 5-aminopentanamidase, and 4-aminobutyrate aminotransferase GabT, respectively, which catalyze the reactions: L-lysine + dioxygen → 5-aminopentanamide + CO2 + H2O, 5-aminopentanamide + H2O → 5-aminopentanoate + ammonium 5-aminopentanoate + 2-oxoglutarate ↔ L-glutamate + glutarate semialdehyde Constitutes the Lysine to 5-oxopentanoate pathway
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 269
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1358
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1518
Illegal AgeI site found at 925
Illegal AgeI site found at 1174
Illegal AgeI site found at 2896 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |