Coding
Part:BBa_K4724006
Designed by: Qingqing zheng Group: iGEM23_NJTech-China-A (2023-10-06)
IsPETaseS93_I94insE-6xHis Tag
We chose this site because that the active pocket amino acids are changed, altering the tertiary structure of the protein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 56
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 56
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 793
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 56
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 56
Illegal AgeI site found at 549 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |