Terminator

Part:BBa_K4800001

Designed by: Yifei Wu   Group: iGEM23_NJTech-China-B   (2023-10-04)
Revision as of 11:45, 4 October 2023 by ZQM 0907 (Talk | contribs)


rrnB T1 terminator

Carboxylate reductase from Mycobacterium marinum(Mutation of glutamine at position 302 to glutamate)



Document

Fig.1 Catalytic pocket for MmCAR

MmCAR is a carboxylic acid reductase from marine bacteria. During the experiment, we mutated the original 302nd amino acid glutamine to glutamic acid through acid-base mutation. The mutated Q302E has a significant improvement compared to the original one

Result:

Fig.2 Q302E and MmCAR produce 1,5-PDO in KA30

Through fermentation experiments, we have verified that Q302E can produce more pentanediol

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None