Coding

Part:BBa_K4800015

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-06)
Revision as of 13:14, 6 October 2023 by Registry (Talk | contribs)

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GabT

The promoter is Ptrc, which can be expressed in Escherichia coli KA30. YciA-sfp-MmCAR is derived from BBa_K1655000. The carboxylic acid reductase MmCAR position 302 is mutated with acid-base, and glutamine is replaced with glutamic acid. Obtain YciA-sfp-MmCAR (Q302E), improve the efficiency of carboxylic acid reduction, and YahK is an aldehyde reductase. This fragment constructs the 5-hydroxyvaleric acid to 1,5-pentanediol pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 328
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None