Coding

Part:BBa_K4677003

Designed by: Yiming Ma   Group: iGEM23_HSASNU   (2023-10-06)
Revision as of 07:53, 6 October 2023 by Registry (Talk | contribs)

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TorT-TorS

The TMAO reductase pathway is controlled by a receptor system that comprises the TMAO-binding protein TorT, the sensor histidine kinase TorS, and the response regulator TorR. TorT associates as a coreceptor with the histidine kinase receptor TorS to form the sensor complex. TorT-TorS is an asymmetric 2:2 complex that binds TMAO with negative cooperativity to form a symmetric active kinase. TMAO-stimulated TorS phosphorylates the response regulator TorR; phosphorylated TorR activates transcription from the torCAD operon to express TorCAD which are involved in the TMAO reductase pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1361
    Illegal XhoI site found at 3615
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 42
    Illegal AgeI site found at 3366
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None