Part:BBa_K4789001

miR-145-sponge

Usage and Biology

LncRNAs, that share miRNA response elements (MREs) with coding RNAs, contained similar miRNA target sequences and then prevented miRNAs from acting on mRNAs. These lncRNAs, known as competing endogenous RNAs (ceRNAs), served as ‘sponges’ for miRNAs, inhibited the corresponding miRNAs and contributed to enhanced translations of their target mRNAs [1](Fig 1). Currently, Reports have shown that the lncRNA MALAT1 can act as a "sponge" for miR-202-3p, thereby regulating the invasion and epithelial-mesenchymal transition of cervical cancer cells [2]. Additionally, it has been reported that MALAT1 can also act as a "sponge" for miR-429, thereby regulating the migration, apoptosis, and other metabolic activities of cervical cancer cells [3-4].

                        
                        Figure 1 Molecular mechanisms of lncRNA-miRNA axes.
 1. MiRNAs regulate gene expression by binding to 3’ UTR elements in mRNA and degrading them.
 2.lncRNAs have been thought to interact with miRNAs as “sponges” or competing  endogenous RNAs (ceRNA), attenuating the 
 repression of mRNAs by miRNA.

Design

Mir-145 affected aerobic glycolysis through MYC, which may be a potential target for the treatment of cervival cancer[5]. Mir-145 contained the binding site of lncRNA MALAT1.Given the fact that lncRNA MALAT1 could act as miRNA sponge, we designed the sequence that could bind to miR-145 based on MALAT1 sequence.

Characterization

The miR-145-sponge sequence was confirmed by Sanger sequencing.

              
                
                 Figure 3 miR-145-sponge validated by Sanger sequencing

Reference

1.Han Xiting,Wang Qian,Wang Yan et al. Long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1/microRNA-202-3p/periostin axis modulates invasion and epithelial-mesenchymal transition in human cervical cancer.[J] .J Cell Physiol, 2019, 234: 14170-14180.

2.Shen Fujin,Zheng Hongyun,Zhou Limei et al. Overexpression of MALAT1 contributes to cervical cancer progression by acting as a sponge of miR-429.[J] .J Cell Physiol, 2019, 234: 11219-11226.

3.Aftab Mehreen,Poojary Satish S,Seshan Vaishnavi et al. Urine miRNA signature as a potential non-invasive diagnostic and prognostic biomarker in cervical cancer.[J] .Sci Rep, 2021, 11: 10323.

4.Chen Mingming,Ma Zhao,Wu Xiaotian et al. A molecular beacon-based approach for live-cell imaging of RNA transcripts with minimal target engineering at the single-molecule level.[J] .Sci Rep, 2017, 7: 1550.

5.Hu Chenchen,Liu Tianyue,Zhang Wenxin et al. miR-145 inhibits aerobic glycolysis and cell proliferation of cervical cancer by acting on MYC.[J] .FASEB J, 2023, 37: e22839.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]