Coding

Part:BBa_K4614002

Designed by: Miaodi Feng   Group: iGEM23_CAU-China   (2023-09-30)
Revision as of 11:38, 30 September 2023 by Registry (Talk | contribs)

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Lnak-R5 


We used T7 promoter to induce IPN and R5(part BBa_K461400) expression, IPN, a carrier protein from the genome of Pseudomonas syringae, used to display R5 on the surface of bacteria.

In use, the modified E. coli can be cultured to the logarithmic stage, and IPTG with a final concentration of 1.0 ug/mL can be added to induce 3 h, and protein expression can be completed

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 529
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 529
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 529
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 529
    Illegal NgoMIV site found at 72
    Illegal NgoMIV site found at 405
    Illegal AgeI site found at 679
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None