Plasmid_Backbone

Part:BBa_K4579033

Designed by: Alexa Morton   Group: iGEM23_Austin-UTexas   (2023-10-05)
Revision as of 21:09, 5 October 2023 by Alexakmorton (Talk | contribs)


pBTK1028 assembly backbone

Following the part type standard of the Bee Toolkit, pBTK1028 is a type 5-1 entry vector part that can be used to create assemblies by combining with type 2, 3, and 4 parts according to the BTK standard. This backbone was created for the BTK by Shaunak Kar, with permission for the UT iGEM team to use it as a basis for our composite part assemblies.

The first four bases of this part correspond to a type 5 part forward prefix, and the last four bases of this part correspond to a type 1 part reverse suffix.
File:Btk-overhangs-low-ish-res.png

For additional info on the BTK standard, see: Leonard, S. P., Perutka, J., Powell, J. E., Geng, P., Richhart, D. D., Byrom, M., ... & Barrick, J. E. (2018). Genetic engineering of bee gut microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids. ACS Synthetic Biology, 7(5), 1279-1290.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2237
    Illegal XbaI site found at 1229
    Illegal XbaI site found at 2246
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2237
    Illegal NheI site found at 2071
    Illegal NotI site found at 182
    Illegal NotI site found at 2225
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2237
    Illegal BglII site found at 1070
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2237
    Illegal XbaI site found at 1229
    Illegal XbaI site found at 2246
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2237
    Illegal XbaI site found at 1229
    Illegal XbaI site found at 2246
    Illegal NgoMIV site found at 105
    Illegal NgoMIV site found at 406
    Illegal AgeI site found at 1661
    Illegal AgeI site found at 1985
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 346
    Illegal SapI site found at 556


[edit]
Categories
Parameters
None