Regulatory

Part:BBa_K4583005

Designed by: Suiru Lu, Chao Tang   Group: iGEM23_SDU-CHINA   (2023-10-04)
Revision as of 18:53, 5 October 2023 by Lusuiru (Talk | contribs)


Pfic

Pfic is a wild-type promoter isolated from Gordonia sp. IITR100.

Usage and Biology

Constitutive gene expression affects growth by imposing a metabolic burden on the cell. Inducible gene expression systems are usually controlled by specific inducers. Inducers are expensive and often lead to irregular protein expression. To overcome this limitation, self-inducible promoters have been developed. The stationary phase promoter isolated from Gordonia sp. IITR100 does not require additional chemical inducers.

  • Stationary Phase Promoter
  • self-inducible
  • Biosafety


Characterization

Protocols

Characterizing using GFP in 2-plasmids Bacteria

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
None