Coding

Part:BBa_K4586030

Designed by: Mohamed Mohamed Saad Aboelghare   Group: iGEM23_AFCM-Egypt   (2023-10-02)
Revision as of 07:09, 4 October 2023 by Mohamed-Ab0elghare (Talk | contribs) (→‎Description)


Citrullinated vimentin

Description

It is an intermediate filament protein that is citrullinated by peptidyl arginine deiminase 2 (PAD2), which converts arginine amino acids into citrulline after translation. It has been demonstrated that citrullinated vimentin has a role in ACPA synthesis, which is a marker for numerous autoimmune diseases, specifically rheumatoid arthritis.

Usage

We initially used this part in our design to be the ligand binding domain in the extracellular part of our Syn notch receptor to interact with the auto-reactive B-cell receptor that secrete ACPA and transmit the signal of this selective binding inside the cell to express our therapeutic cargo within the modified exosomes that target the auto reactive B-cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None