Coding

Part:BBa_K4907000

Designed by: Yannan You   Group: iGEM23_XMU-China   (2023-09-09)
Revision as of 04:56, 5 October 2023 by Yank (Talk | contribs) (→‎Biology)


sfibp-his tag


Biology

k4907000.png

sfibp

SfIBP is a kind of antifreeze proteins (AFPs) found from Shewanella frigidimarina (1). It is capable to prevent liquid from freezing within a certain temperature range and inhibit ice growth, which is defined as Thermal Hysteresis (TH) and ice recrystallization inhibition (IRI) respectively. Additionally, the IRI activity of SfIBP is much higher beyond other AFPs.

Usage and Design

In order to measure TH and IRI activity of SfIBP, and finally verify if it is capable to solve soil frost problem, a His-tag (6×his) was added to the C-terminal of SfIBP for purification. We constructed this part and assembled it on the expression vector pET-28a(+).

123

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 634
    Illegal AgeI site found at 649
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None