Composite
Part:BBa_K4585009
Designed by: Zijun Zhang Group: iGEM23_CSU-CHINA (2023-10-04)
pcDNA3.1(+)-3xHA-Gal4-VP64-Degron-NLS linearized vector
The objective is to perform homologous recombination with the Degron homologous recombination fragment , The synthetic pcDNA3.1 (+) -3 HA-Gal 4-VP64-Degron-NLS plasmid (BBa _ K4585015) was used for subsequent experiments.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 25
Illegal XbaI site found at 64
Illegal XbaI site found at 6170
Illegal SpeI site found at 4828
Illegal PstI site found at 30
Illegal PstI site found at 1459 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 25
Illegal SpeI site found at 4828
Illegal PstI site found at 30
Illegal PstI site found at 1459
Illegal NotI site found at 51 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 25
Illegal BglII site found at 4591
Illegal BamHI site found at 6027
Illegal BamHI site found at 6216
Illegal XhoI site found at 58
Illegal XhoI site found at 5797 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 25
Illegal XbaI site found at 64
Illegal XbaI site found at 6170
Illegal SpeI site found at 4828
Illegal PstI site found at 30
Illegal PstI site found at 1459 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 25
Illegal XbaI site found at 64
Illegal XbaI site found at 6170
Illegal SpeI site found at 4828
Illegal PstI site found at 30
Illegal PstI site found at 1459
Illegal NgoMIV site found at 569
Illegal NgoMIV site found at 1910
Illegal NgoMIV site found at 2195
Illegal AgeI site found at 122 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5716
Illegal BsaI.rc site found at 3723
Illegal BsaI.rc site found at 5461
Illegal SapI site found at 2640
Illegal SapI.rc site found at 1759
Illegal SapI.rc site found at 1969
[edit]
Categories
Parameters
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