Plasmid
PrtDEF

Part:BBa_K258008:Design

Designed by: Jung Hoon Ahn from Korea Advanced Institute of Science and Technology   Group: iGEM09_METU-Gene   (2009-10-10)
Revision as of 01:16, 11 October 2009 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 210
    Illegal PstI site found at 793
    Illegal PstI site found at 1140
    Illegal PstI site found at 3055
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4344
    Illegal PstI site found at 210
    Illegal PstI site found at 793
    Illegal PstI site found at 1140
    Illegal PstI site found at 3055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1259
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 210
    Illegal PstI site found at 793
    Illegal PstI site found at 1140
    Illegal PstI site found at 3055
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 210
    Illegal PstI site found at 793
    Illegal PstI site found at 1140
    Illegal PstI site found at 3055
    Illegal NgoMIV site found at 4706
    Illegal AgeI site found at 3189
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used whole plasmid in order to excrete a fusion protein with one of two signal tags which are TliA and LARD1


Source

Sequence and Features PrtDEF: ABC transporter of Erwinia chrysanthemi for PrtA EGF: Human Epidermal Growth Factor

References