Plasmid
PrtDEF
Part:BBa_K258008:Design
Designed by: Jung Hoon Ahn from Korea Advanced Institute of Science and Technology Group: iGEM09_METU-Gene (2009-10-10)
Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 210
Illegal PstI site found at 793
Illegal PstI site found at 1140
Illegal PstI site found at 3055 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4344
Illegal PstI site found at 210
Illegal PstI site found at 793
Illegal PstI site found at 1140
Illegal PstI site found at 3055 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1259
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 210
Illegal PstI site found at 793
Illegal PstI site found at 1140
Illegal PstI site found at 3055 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 210
Illegal PstI site found at 793
Illegal PstI site found at 1140
Illegal PstI site found at 3055
Illegal NgoMIV site found at 4706
Illegal AgeI site found at 3189 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used whole plasmid in order to excrete a fusion protein with one of two signal tags which are TliA and LARD1
Source
Sequence and Features PrtDEF: ABC transporter of Erwinia chrysanthemi for PrtA EGF: Human Epidermal Growth Factor