Regulatory

Part:BBa_K4583000

Designed by: Suiru Lu, Chao Tang   Group: iGEM23_SDU-CHINA   (2023-10-04)
Revision as of 11:55, 4 October 2023 by Liang QF (Talk | contribs) (→‎Protocols)

PYU3

PYU3 is the promoter of the gene orf-0464, and it comes from Escherichia coli. It will express at the late stationary phase.

Usage and Biology

When the bacteria enter the stationary phase, the physiological state of the bacteria changes significantly. During this phase, many genes will respond to make timely adjustments. This part (BBa_K4583000) is the promoter of the gene orf-0464. Its most notable feature is that it will be expressed in the late stationary phase. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

Protocols

Experiment Conditions

  • E. coli MG1655
  • 30oC, 48h
  • Equipment:

Data Curation

Characterization using fluorescent proteins

Comparison of expression time with cell density

[edit]
Categories
//chassis/prokaryote/ecoli
//promoter
Parameters
None